Biochemistry
Omics_Microbes:
Introduction
Sepsis (also known as blood poisoning) is the immune system’s overreaction to an infection. Normally the human immune system fights infection but sometimes it attacks our body’s own organs and tissues. If not treated immediately, sepsis can result in organ failure and death. Yet with early diagnosis, it can be treated with antibiotics. Five people die each hour in the UK as a result of sepsis.
Study Design
Blood plasma samples were collected, processed and frozen from subjects visiting the X Hospital Accident and Emergency Unit and who were diagnosed with sepsis. Age and gender-matched control subjects were recruited from the same Accident and Emergency Unit and blood plasma was also collected, processed and stored; these control subjects were tested for but were not diagnosed with sepsis. The sepsis and control samples have been analysed applying a quantitative proteomics method; a specific protein has received a more in-depth mass spectrometry analysis; and the sepsis and control samples have been analysed applying an untargeted metabolomics method to identify changes in metabolites.
Assessment
This assessment will allow you to investigate the data acquired. The assessment is broken down in to three separate sections.
The assessment should be submitted as a short scientific report defining (a) the computational methods applied; (b) the data analysis results generated and (c) a discussion/conclusion section. The maximum word count is 800 words.
The data collected is presented in the file named ‘Proteomics data.xls’.
- Construct a volcano plot to visualise the changes in fold change and p values from health to sepsis. (By excel)
- If you assume p<0.05 is significant and prioritise two-fold increased or decreased: name the two most increased and two most decreased proteins of significant interest.
- What are the biological processes for these proteins?
Serum samples were extracted and analysed for 15 subjects diagnosed with sepsis and 15 subjects not diagnosed with sepsis. Plasma was extracted in 50/50 methanol/water (v/v). Each sample was analysed applying a ultra-performance liquid chromatography-mass spectrometry (LC-MS) method followed by raw data processing. Quality Control (QC) samples were also analysed as part of the study.
The processed LC-MS data is presented in the file named ‘Omics_Microbes_Metabolomics_dataset_Case_Study.csv’.
- Assess the quality of the data provided applying multivariate analysis methods. Define whether the data is of an adequate quality to perform statistical analysis.
- Applying univariate statistical analysis methods, identify the metabolites which show a statistical significance between subjects with sepsis and subjects without sepsis. Report the metabolite and statistical critical p-value for all metabolites with a critical p-value < 0.05 (FDR-corrected).
- Identify the metabolite pathway or pathways that are associated with the statistically significant metabolites described in part b.
Custompaper.help is an academic writing platform that provides students and professionals with quality services to ease their assignment challenges. With a wide range of services, the platform has become one of the leading academic writing service providers in the market due to its reliability and affordability. Below, we shall discuss some of the services which Custompaper.help provides:
Essay Writing Service
The essay writing service provides customers with quality essays on any subject or topic they requested within a given deadline. The writers at custompaper are experts in their respective fields, and they ensure customer satisfaction by producing original content with thorough research involved backed up with strong evidence from reliable sources available online or offline. All essays come with free plagiarism reports as well as revisions if needed so that customers can trust that they are getting high-quality work for their money’s worth.
Research Paper Writing Service
This is another popular service offered by custom paper help wherein customers are assisted in conducting research papers according to their needs and requirements within specified deadlines. Writers at custom paper help have vast knowledge about various topics belonging to different disciplines such as Management, Arts & Humanities, History etc., allowing them to craft impeccable research papers every time regardless of the complexity or urgency level requested by customers. Additionally, all our research papers come along with proper citation formats which ensures customer’s safety when it comes to plagiarism issues while also helping them save time on formatting procedures thus improving overall efficiency even further!
Dissertation Writing Service
CustomPaperHelp also offers dissertation writing assistance for those who need it most – students preparing for graduation or professionals wanting to further refine their skills through higher education levels such as Master/PhD degrees etc, Our team of writers consists of experienced individuals having Master’s/PhD degrees themselves so you can rest assured that your dissertation will be handled expertly from the start till the end! Furthermore, we offer unlimited revisions until your complete satisfaction is achieved, along with regular checkups being done throughout the process just so everything goes according to course without any hiccups!
Editing And Proofreading Services
If you already have written an essay but want professional feedback before submitting it, then CustomPaperHelp also offers editing & proofreading services which helps in identifying & rectifying errors present in assignments like grammar mistakes, spelling mistakes, punctuation problems etc., thereby ensuring top-notch quality work, before submission takes place! We understand how important accuracy & consistency are when it comes to grading systems used at universities nowadays, so taking extra care during this phase becomes absolutely essential if good grades are desired afterward!
Coursework Help
For those who find coursework difficult due to complications of being quite technical in nature; CustomPaperHelp extends its support here too, by providing quality solutions every single time – starting from understanding concepts properly up until composing flawless answers following university guidelines closely enough making sure no loopholes remain whatsoever! Whether it’s related math problem-solving algorithms or lengthy home assignments – our expert panel has got you covered from head-to-toe leaving no stone unturned during this very crucial stage since final grades depend largely upon the success rate achieved here successfully!
Assignment Help
Assignments form an integral part of getting better scores & ultimately, a degree itself (in case pursuing a masters/PhD) – something only possible if precision is made a priority right away; otherwise, chances of failure increase exponentially over a time period detrimentally affecting performance evaluation systems later down road drastically hence stressing timely importance completion prior commencement other operations associated same project altogether consistently meeting high standards expected each situation respectively! Here too, CustomPaperHelp presents comprehensive solution package exceeding expectations beyond point offering optimal values therein ensuring maximum satisfaction reached under circumstances prevailing accordingly without fail…proving once more why people trust us implicitly without second thought whatsoever!!
