Having made it through Cell Molecular Biology this far, you are now an expert on cells and their molecular mechanisms. There are approximately 10,000 proteins in a typical cell that are synthesized through different routes of intracellular protein trafficking. Each of you will be given a different protein and its final location in the cell. Starting from the mRNA that is produced and released into the cytosol, describe in detail how the protein is translated and where this occurs. Additionally, describe how the protein is being processed and where, along with how it is being transported or further processed until it reaches its final destination.
Depending on your protein, many will have very different routes that they follow. Provide enough detail to complete 2 – 3 pages. This can be done by focusing in detail the more complex steps; What mode(s) of protein trafficking occurs ? Is your protein glycosylated? Does it have disulfide bonds? If it goes to the nucleus, what size is the protein? If it simply stays in cytosol, then you will need to provide more detail about its transcription and translation than given in class. If it goes to several organelles, just focus detail on one part of the process.
If you are having trouble with this assignment, contact me. However, do your best at trying on your own first so a meaningful discussion can occur.
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