Here is an outline of what my topic is supposed to be about. My paper should elucidate how this emerging technology
can protect public health from an environmental health perspective:
INTRO: Describe what LAMP is and how it is used to detect Salmonella in various food matrices.
METHODS: How does LAMP work? What steps are taken to run a LAMP sample to test it for Salmonella?
RESULTS: How do you interpret the results from a LAMP sample to test it for Salmonella?
DISCUSSION: Why is LAMP better or worse for detecting Salmonella from food?
*Sensitivity/Specificity of the assay.
*Limit of Detection, and compare these measures to PCR or other methods used to traditionally detect
Salmonella!
CONCLUSION: Summarize why LAMP is used and how this technology can protect public health.
This is what I have so far:
1. Salmonella– is a bacteria that causes infection to humans by eating contaminated foods with animal feces. Salmonella has been in meat products, poultry products, raw or undercooked eggs, and dough, fruits, and dairy products. The Rapid Microbiological Method that scientists have used is called Loop-mediated isothermal amplification (LAMP). This mechanism is an alternative to a polymerase chain reaction (PCR) for pathogen detection in clinical specimens and food matrices.
2. A major advantage of LAMP over PCR is the high tolerance to biological substances, such as whole blood and urine, commonly found in clinical specimens. Another advantage of LAMP is that it has shown a greater tolerance to potential assay inhibitors (e.g. soil, culture media, humic acid) than PCR. Salmonella LAMP assays have been used in a wide variety of food matrices, including all the major food categories linked to Salmonella outbreak-associated illnesses, for example, produce, eggs, chicken, pork, and beef. This method would make an impact on environmental health because this method is reliable, robust, and can help detect Salmonella in order to prevent foodborne illnesses.
References:
Fang J, Wu Y, Qu D, et al. Propidium monoazide real-time loop-mediated isothermal amplification for specific visualization of viable Salmonella in food. Lett Appl Microbiol 2018. [Epub ahead of print].
Hu L, Ma LM, Zheng S, et al. Development of a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Salmonella ser. Enteritidis from egg products. Food Control 2018;88:190197. Crossref, Google Scholar
CDC. Reports of Selected Salmonella Outbreak Investigations. 2018.
Here are additional links to use for the essay. This will help to discuss the sensitivity/specificity of the assay.
1. https://www.fws.gov/aah/PDF/QI-Terms%20and%20Defs.pdf
2. https://www.fws.gov/aah/PDF/SandS.pdf
3. https://pubmed.ncbi.nlm.nih.gov/32964461/
4.https://www.fws.gov/aah/PDF/QI-Terms%20and%20Defs.pdf
Why Work with Us
Top Quality and Well-Researched Papers
We always make sure that writers follow all your instructions precisely. You can choose your academic level: high school, college/university or professional, and we will assign a writer who has a respective degree.
Professional and Experienced Academic Writers
We have a team of professional writers with experience in academic and business writing. Many are native speakers and able to perform any task for which you need help.
Free Unlimited Revisions
If you think we missed something, send your order for a free revision. You have 10 days to submit the order for review after you have received the final document. You can do this yourself after logging into your personal account or by contacting our support.
Prompt Delivery and 100% Money-Back-Guarantee
All papers are always delivered on time. In case we need more time to master your paper, we may contact you regarding the deadline extension. In case you cannot provide us with more time, a 100% refund is guaranteed.
Original & Confidential
We use several writing tools checks to ensure that all documents you receive are free from plagiarism. Our editors carefully review all quotations in the text. We also promise maximum confidentiality in all of our services.
24/7 Customer Support
Our support agents are available 24 hours a day 7 days a week and committed to providing you with the best customer experience. Get in touch whenever you need any assistance.
Try it now!
How it works?
Follow these simple steps to get your paper done
Place your order
Fill in the order form and provide all details of your assignment.
Proceed with the payment
Choose the payment system that suits you most.
Receive the final file
Once your paper is ready, we will email it to you.
Our Services
No need to work on your paper at night. Sleep tight, we will cover your back. We offer all kinds of writing services.
Essays
No matter what kind of academic paper you need and how urgent you need it, you are welcome to choose your academic level and the type of your paper at an affordable price. We take care of all your paper needs and give a 24/7 customer care support system.
Admissions
Admission Essays & Business Writing Help
An admission essay is an essay or other written statement by a candidate, often a potential student enrolling in a college, university, or graduate school. You can be rest assurred that through our service we will write the best admission essay for you.
Reviews
Editing Support
Our academic writers and editors make the necessary changes to your paper so that it is polished. We also format your document by correctly quoting the sources and creating reference lists in the formats APA, Harvard, MLA, Chicago / Turabian.
Reviews
Revision Support
If you think your paper could be improved, you can request a review. In this case, your paper will be checked by the writer or assigned to an editor. You can use this option as many times as you see fit. This is free because we want you to be completely satisfied with the service offered.